Introduction:
QPCR(Polymerase Chain Reaction) gives you a choice of quantitative methods for gene expression.The NB988 Real-time PCR is very useful and important for life science research and curative evaluation, and it is innovative and pioneering products in Lab. for the prevention, diagnosis, evaluation and monitoring of diseases.
Key Features:
*48 Samples
*Easy to use Software
*Various Operation Modes
*Advanced Temperature Control
*Non-maintenance Optical System
*Sensitive and Precise Thermoelectric Systems
*Particular Momentary Power Failure Protection
Technical parameters:
Thermal Block
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Block formats
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48-well
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Sample volume
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10-100ul
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Consumables
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Thick-walled0.2ml PCR tubes,8-strip tubes
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Max. heating rate
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3.0℃/s
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Max. Cooling rate
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2.0℃/s
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Temperature range
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4.0-99.0℃
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Temperature accuracy
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±0.1℃
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Temperature uniformity
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±0.3℃
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Temperature steps
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1-99
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Heat Lid
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Temperature range
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100-120℃
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Optics
|
|
Excitation
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2LEDS
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Excitation range
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470-523nm
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Detector
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PMT(Photomultipier tubes)
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Detection range
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525-564nm
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Detection channels
|
|
Channel
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Excitation(nm)
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Emission(nm)
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Pre.Calibrated Dyes
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1
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470
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525
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FAM,SYBR CREENI
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2
|
523
|
564
|
VIC,HEX,TET,JOE
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Detection range
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1-10^10copies
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SOFTWARE
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Analysis modes
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Absolute quantification,Relative quantification,Melt curve analysis
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PERFORMANCE
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Reproducibility Correlation coefficient of nucleic acid precision
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CV≤2.1%
R≥0.997
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SYSTEM
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Operating systems
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Windows XP,Windows 7
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Communication
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USB
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Power usage
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AC220*(1±10%)V,50*(1±2%)Hz
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Dimensions
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520mm*420mm*320mm
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Weight
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27kg
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Heating & cooling unit:
Applying the advantages of thermoelectric (TE, Peltier Effect) module makes temperature change rapidly and uniform. Hot-lid design permits oil-free operation.
Optical unit
An array of LED collimates to corresponding wells.High-light and long-lived LED excitation light source does not need maintenance or preheating.
PMT fluorescence detection offers high sensitivity, broad dynamic range and low crosstalk.
An array of detecting coaxial optical fibers focus on each well’s bottom ensure minimal light loss.
Operation system and Application software:
Application software interface is friendly windows style, Monitoring real-time amplification plots and data analysis during a PCR run.
Supply software package of medical case and database management and plot display.
Quantification:
After quantitative analysis for the standard sample, the instrument will get a standard curve of Ct value related to copy number.
Comparing the Ct value of unknown sample with standard curve, the instrument can calculate the original copy number of unknown sample by quantitative analysis. NB988 has a wide linear detecting range, which can detect 101-1010 DNA copies/ml.