PCR is an extremely important tool for molecular biology research, and is a molecular biology technique used to amplify specific DNA fragments. Real-time fluorescent quantitative PCR system adopts fluorescent quantitative PCR technology, that is, performs fluorescence detection on the basis of PCR technology.

Application scenarios of real-time fluorescent quantitative PCR system:

1. People's hospitals at all levels, maternal and child health care centers, dental hospitals, children's hospitals, blood banks and other medical institutions, and disease prevention and control centers at all levels, etc. use real-time fluorescent quantitative PCR instruments for clinical molecular diagnosis.

2. Animal disease prevention and control centers, agricultural and rural bureaus, animal husbandry and veterinary stations, large-scale breeding enterprises, pet hospitals, etc. use real-time fluorescent quantitative PCR instruments for animal health (animal infectious diseases and pet medical care) testing.

3. Food and medicine administrations at all levels, customs at all levels, self-inspection of food companies, third-party testing companies, quick inspection rooms, etc. use real-time fluorescent quantitative PCR instruments for food safety (including medicine and cosmetics) testing.

The real-time fluorescent quantitative PCR machine is composed of a fluorescent quantitative system and a computer, which is used to monitor the fluorescence in the circulation process. A computer linked to a real-time device collects fluorescence data, and the data are displayed in the form of graphs through the developed real-time analysis software. Original data are plotted as a graph of fluorescence intensity versus cycle number, and the analysis can begin after original data collection.