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Tips for selecting fluorescence quantitative PCR system
2024-02-27 14:39

The fluorescence quantitative PCR system is a commonly used instrument in molecular biology experiments, used to detect and quantify DNA or RNA molecules. When choosing a fluorescence quantitative PCR system, here are some factors to consider:

gradient thermal cycler pcr 

1. Sensitivity and accuracy: The PCR machine's ability to detect trace amounts of DNA or RNA requires high sensitivity and accuracy to ensure the reliability of experimental results.

2. Temperature control system: The temperature control system of the PCR machine needs to have good stability and be able to accurately control temperature changes to ensure the accuracy and stability of the PCR reaction.

3. PCR amplification throughput: The throughput refers to the number of reaction tubes for the PCR reaction. The appropriate PCR amplification throughput needs to be selected according to the experimental requirements.

4. Convenience of experimental operation: The operating interface and software of the PCR machine need to be easy to operate and meet the operating habits and needs of the laboratory personnel.

5. Cost-effectiveness: Pay attention to the balance between the price and performance of the equipment and choose one with higher cost performance.

real time pcr analyzer 

PCR instrument, also known as thermal cycler and gene amplification instrument, is an instrument that uses PCR (polymerase chain reaction) technology to rapidly amplify specific DNA in vitro. According to the purpose of DNA amplification and detection standards, PCR machines can be divided into four categories: ordinary PCR machines, gradient PCR machines, in-situ PCR machines, and real-time fluorescence quantitative PCR machines.


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