Real-time fluorescence quantitative PCR machine, also known as qPCR machine, can be used to complete PCR reaction and perform real-time quantitative detection and monitoring of the generated fluorescence signal.

Real-time fluorescence quantitative PCR machine can complete PCR amplification and detection in one step, so there is no need to use gel electrophoresis to detect products, and truly realize quantitative PCR. Real-time fluorescence quantitative PCR systems use fluorescent dyes to detect the accumulation of PCR products in the exponential phase of the reaction to achieve rapid and accurate product quantification and target data analysis. In contrast, endpoint PCR requires subjective analysis of products through gel electrophoresis, Northern blot or chip.

qPCR machine has many advantages over these semi-quantitative detection methods, including:

1. Lower risk of contamination

2. Simple operation procedures

3. Ability to obtain results fast

4. Wide dynamic range

5. Higher sensitivity

6. Easy to optimize

7. Cost-effective

8. High throughput

9. Requires less RNA or DNA loading